Regulations for Enforcement of the Slaughterhouse Act（Order of the Ministry of Health and Welfare No. 44 of 1953）.

These Regulations for Enforcement of the Slaughterhouse Act (Order No. 44 of 1953) detail the operational, sanitary, and procedural requirements for slaughterhouses in Japan. It outlines requirements for facility applications, hygiene management, equipment maintenance, and disease prevention. Specific provisions govern water quality, refrigeration, waste disposal, and the handling of livestock and meat to ensure public health. The Regulations also mandate training for sanitation managers and slaughterers, and establish protocols for inspections and documentation.
Additionally, the Regulations define procedures for slaughter outside designated facilities, criteria for disease-related inspections, and requirements for removing animal parts. They includes qualifications for inspectors, and detailed appendices listing diseases, inspection criteria, and seal requirements.